| ncRNA name | RASSF1A siRNA |
| ncRNA Category | siRNA |
| Disease name | uveal melanoma (UM) |
| Species | Homo sapiens |
| Tissues/Cell_line | UM samples, normal scleral/choroidal tissue; human UM cell lines: 92.1, Mel270, OCM-1, Mum2B, TP17, m2, m2F |
| Methods | MTT assay, FISH analysis, LOH analysis, methylation-specific PCR, qRT-PCR, Western blot, siRNA transfection, flow cytometry |
| Expression pattern | associated |
| Functional description | SiRNA-mediated depletion of RASSF1A increased ERK1/2 activation, cyclin D1 expression, and also decreased p27(Kip1) expression in normal uveal melanocytes. The depletion of RASSF1A induced senescence-associated β-galactosidase activity and increased p21(Cip1) expression suggests that RASSF1A plays a role in the escape of cellular senescence in normal uveal melanocytes. |
| PubMed ID | 21788308 |
| Year | 2011 |
| Title | Status of RASSF1A in uveal melanocytes and melanoma cells. |
| Drug-related ncRNA | NO |
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